SPECIFICITY OF IMPROVED METHODS FOR MYCOBACTIN BIOASSAY BY ARTHROBACTER TERREGENS

AUTOR(ES)

Antoine, Alan D.

RESUMO

Antoine, Alan D. (Johns Hopkins University-Leonard Wood Memorial Leprosy Research Laboratory, Baltimore, Md.), Norman E. Morrison, and John H. Hanks. Specificity of improved methods for mycobactin bioassay by Arthrobacter terregens. J. Bacteriol. 88:1672–1677. 1964.—Arthrobacter terregens was used to assay mycobactin, a growth factor for Mycobacterium paratuberculosis. Improved techniques permit the assay of mycobactin within 3 to 4 days by agarplate or liquid-medium methods. For the agarplate method, Arthrobacter terregens gave linear increases in zonal growth at mycobactin concentrations of 0.07 to 0.30 μg per spot; for the liquid-medium method, linear increases in turbidimetric growth occurred at 0.05 to 0.27 μg/ml. Specificity studies show that the mycobactin hydrolytic products, cobactin and mycobactic acid, function as growth stimulators, but the high concentrations required would produce only minimal interference in mycobactin assays. Furthermore, the response to mycobactic acid is characterized by a delayed response of 3 days. Various synthetic hydroxylamine-containing compounds and metalchelating agents cannot replace the biological activity of mycobactin. Diacetylmycobactin is 7.4 times more effective than mycobactin as a growth stimulator.

Documentos Relacionados

• USE OF ARTHROBACTER TERREGENS FOR BIOASSAY OF MYCOBACTIN
• Growth factor activity of mycobactin for Arthrobacter species.
• Synthetic Metal Chelators Which Replace the Natural Growth-Factor Requirements of Arthrobacter terregens
• An Improved Bioassay for Cytokinins Using Cucumber Cotyledons 1
• Improved Methods for Immunoassay of Mycothiol