Spectrofluorometric assay for rapid detection of total and fecal coliforms from surface water.
AUTOR(ES)
Park, S J
RESUMO
With a spectrofluorometer, the length of the incubation time required in the fluorogenic assay was reduced to 12 h. The threshold emissions for reading the fluorogenic reaction by the spectrofluorometer were 5 and 10 U for lauryl tryptose broth media containing 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide, respectively. These two kinds of threshold units were equivalent to known concentrations of free 4-methylumbelliferone of 2.5 and 6 microM, respectively, in lauryl tryptose broth media.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=167470Documentos Relacionados
- Modification of delayed-incubation procedure for detection of fecal coliforms in water.
- New medium for the simultaneous detection of total coliforms and Escherichia coli in water.
- Rapid detection of total and fecal coliforms in water by enzymatic hydrolysis of 4-methylumbelliferone-beta-D-galactoside.
- Rapid enumeration of Fecal Coliforms in water by a colorimetric beta-galactosidase assay.
- Rapid Method for Detection and Enumeration of Fecal Coliforms in Fresh Chicken
