Splicing as a requirement for biogenesis of functional 16S mRNA of simian virus 40.
AUTOR(ES)
Gruss, P
RESUMO
Simian virus 40 deletion mutants were constructed lacking specifically the intervening sequences for a late viral mRNA. The construction method involved the replacement of portions of the late simian virus 40 genes with the DNA segment from reverse transcription of the viral mRNAs. Restriction endonuclease cleavage and sequence analysis confirmed the precise structure of the mutant DNAs and demonstrated that they contained the genetic information for VP1, including all potential 5' ends for the late viral RNAs. Thus, the primary late transcription product(s) of this mutant should have the structure of functional 16S mRNAs. Complementation analysis as well as immunoprecipitation showed, however, that deletion of the intervening sequences from this mutant prevented the expression of VP1. The nature of this failure appears to be a defect in the posttranscriptional processing of the viral RNA. These results indicate that splicing is an essential function in the biogenesis of certain mRNAs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411565Documentos Relacionados
- Mapping of transcription sites of simian virus 40-specific late 16S and 19S mRNA by electron microscopy.
- Rapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase.
- Mutational alterations within the simian virus 40 leader segment generate altered 16S and 19S mRNA's.
- Capping structures of simian virus 40 19S and 16S mRNAs.
- The number of ribosomes on simian virus 40 late 16S mRNA is determined in part by the nucleotide sequence of its leader.