Splicing enhances translation in mammalian cells: an additional function of the exon junction complex
AUTOR(ES)
Nott, Ajit
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
In mammalian cells, spliced mRNAs yield greater quantities of protein per mRNA molecule than do otherwise identical mRNAs not made by splicing. This increased translational yield correlates with enhanced cytoplasmic polysome association of spliced mRNAs, and is attributable to deposition of exon junction complexes (EJCs). Translational stimulation can be replicated by tethering the EJC proteins Y14, Magoh, and RNPS1 or the nonsense-mediated decay (NMD) factors Upf1, Upf2, and Upf3b to an intronless reporter mRNA. Thus, in addition to its previously characterized role in NMD, the EJC also promotes mRNA polysome association. Furthermore, the ability to stimulate translation when bound inside an open reading frame appears to be a general feature of factors required for NMD.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=324426Documentos Relacionados
- The exon junction complex is detected on CBP80-bound but not eIF4E-bound mRNA in mammalian cells: dynamics of mRNP remodeling
- Magoh, a human homolog of Drosophila mago nashi protein, is a component of the splicing-dependent exon–exon junction complex
- Splicing of U12-type introns deposits an exon junction complex competent to induce nonsense-mediated mRNA decay
- An RNA-histone complex in mammalian cells: the isolation and characterization of a new RNA species.
- hnRNP A1 Recruited to an Exon In Vivo Can Function as an Exon Splicing Silencer