Stable association of viral protein VP1 with simian virus 40 DNA.
AUTOR(ES)
Brady, J N
RESUMO
Mild dissociation of simian virus 40 particles releases a 110S virion core nucleoprotein complex containing histones and the three viral proteins VP1, VP2, and VP3. The association of viral protein VP1 within this nucleoprotein complex is mediated at least partially through a strong interaction with the viral DNA. Treatment of the virion-derived 110S nucleoprotein complex with 0.25% Sarkosyl dissociated VP2, VP3, and histones, leaving a stable VP1-DNA complex. The VP1-DNA complex had a sedimentation value of 30S and a density of 1.460 g/cm3. The calculated molecular weight of the complex was 7.9 x 10(6), with an average of 100 VP1 molecules per DNA. Agarose gel electrophoresis of the VP1-DNA complex demonstrated that VP1 is associated not only with form I and form II simian virus 40 DNAs but also with form III simian virus 40 DNA generated by cleavage with EcoRI.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=171352Documentos Relacionados
- Simian virus 40 mutant with transposed T-antigen and VP1 genes.
- Role of Simian Virus 40 Vp1 Cysteines in Virion Infectivity
- Vp1 affects intracellular localization of Vp3 polypeptide during simian virus 40 infection.
- Association with capsid proteins promotes nuclear targeting of simian virus 40 DNA.
- Suppression of a VP1 mutant of simian virus 40 by missense mutations in serine codons of the viral agnogene.