Staphylococcal Enterotoxin Synthesis During the Exponential, Transitional, and Stationary Growth Phases

AUTOR(ES)

Czop, Joyce K.

RESUMO

Small inocula (1 to 10 colony-forming units per ml of broth) of Staphylococcus aureus strains S-6, S-6R, and FRI-100 were employed to study growth and enterotoxin synthesis in 4% protein hydrolysate powder broths. For each strain, the exponential growth phase ended once the population approached 109 to 2 × 109 colony-forming units per ml. By that time, the concentrations of enterotoxins A and B reached the minimal level (1 to 2 μg/ml) at which the single gel diffusion tube method becomes applicable. By microslides and reverse passive hemagglutination, enterotoxins A and B were found to be synthesized during the exponential growth phase, but at different exponential rates.

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