Stepwise formation of P-cluster in nitrogenase MoFe protein
AUTOR(ES)
Lee, Chi Chung
FONTE
National Academy of Sciences
RESUMO
The P-cluster of nitrogenase is one of the most complex biological metallocenters known to date. Despite the recent advances in the chemical synthesis of P-cluster topologs, the biosynthetic mechanism of P-cluster has not been well defined. Here, we present a combined biochemical, electron paramagnetic resonance, and X-ray absorption spectroscopy/extended X-ray absorption fine-structure investigation of the maturation process of P-clusters in ΔnifH molybdenum-iron (MoFe) protein. Our data indicate that the previously identified, [Fe4S4]-like cluster pairs in ΔnifH MoFe protein are indeed the precursors to P-clusters, which can be reductively coupled into the mature [Fe8S7] structures in the presence of Fe protein, MgATP, and dithionite. Moreover, our observation of a biphasic maturation pattern of P-clusters in ΔnifH MoFe protein provides dynamic proof for the previously hypothesized, stepwise assembly mechanism of the two P-clusters in the α2β2-tetrameric MoFe protein, i.e., one P-cluster is formed in one αβ dimer before the other in the second αβ dimer.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2774011Documentos Relacionados
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