Sterilization of the biological safety cabinet

AUTOR(ES)

Newsom, S. W. B.

RESUMO

Test strips comprising B subtilis spores or M phlei cells dried on aluminium foil (some serum-coated) were used to test sterilizing processes currently advised for safety cabinets. Exposure to formalin from a bowl, boiling excess formalin with the fan running, and ultraviolet radiation were all found to be unreliable, as was glutaraldehyde. Vaporization of 2 ml of formalin per 0·028 m3 followed by exposure for 18 hours at ambient temperatures was 100% successful, and the depolymerization of paraformaldehyde tablets with a hair drier was a simple, very effective alternative method.

Documentos Relacionados

• Modified Laminar Flow Biological Safety Cabinet
• A test system for the biological safety cabinet
• Influence of crossdrafts on the performance of a biological safety cabinet.
• Evaluation of a class III biological safety cabinet for enclosure of an ultracentrifuge.
• Use of the microscope in a gastight safety cabinet system.