Stf1: Non-Wee Mutations Epistatic to Cdc25 in the Fission Yeast Schizosaccharomyces Pombe
AUTOR(ES)
Hudson, J. D.
RESUMO
In Schizosaccharomyces pombe, cdc25 is a cell cycle regulated inducer of mitosis. wee1 and phenotypically wee alleles of cdc2 are epistatic to cdc25. Mutant alleles of a new locus, stf1 (suppressor of twentyfive), identified in a reversion analysis of conditionally lethal cdr1-76 cdc25-22 and cdr2-96 cdc25-22 double mutant strains, also suppress both temperature-sensitive and gene disruption alleles of cdc25. These mutants, by themselves, are phenotypically indistinguishable from wild type strains; hence they represent the first known mutations that are epistatic to cdc25 and do not display a wee phenotype. stf1 genetically interacts with other elements of mitotic control in S. pombe. stf1-1 is additive with wee1-50, cdc2-1w and cdc2-3w for suppression of cdc25-22. Also, like wee1(-) and cdc2-w, stf1(-) suppression of cdc25 is reversed by overexpression of the putative type 1 protein phosphatase bws1(+)/dis2(+). Interaction with various mutants and plasmid overexpression experiments suggest that stf1 does not operate either upstream or downstream of wee1. Similarly, it does not operate through cdc25 since it rescues the disruption. stf1 appears to encode an important new element of mitotic control.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1204185Documentos Relacionados
- Stockpiling of Cdc25 during a DNA replication checkpoint arrest in Schizosaccharomyces pombe.
- Modeling the fission yeast cell cycle: Quantized cycle times in wee1− cdc25Δ mutant cells
- Spatial organization of the Nim1-Wee1-Cdc2 mitotic control network in Schizosaccharomyces pombe.
- Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2.
- The Wee1 protein kinase regulates T14 phosphorylation of fission yeast Cdc2.