Stimulation of in vitro transcription from heterologous promoters by the simian virus 40 enhancer.
AUTOR(ES)
Sassone-Corsi, P
RESUMO
Insertion of the simian virus 40 enhancer upstream from the +33 to -34 adenovirus major late promoter element or the +62 to -102 conalbumin promoter region causes a 10-fold stimulation of specific transcription using a whole cell extract but not an S100 extract. Many of the in vivo effects of the enhancer were mimicked in vitro. This stimulation occurred only in cis, with either orientation of the 72-base-pair repeat, and was markedly decreased by deletions known to diminish the enhancer activity in vivo. However, in vitro, the 72-base-pair repeat did not stimulate at a distance or in the presence of a strong upstream promoter element.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=344665Documentos Relacionados
- Stereoalignment requirements for activation of transcription by the simian virus 40 enhancer.
- trans Activation of the simian virus 40 enhancer.
- Cell-specific activity of the constituent elements of the simian virus 40 enhancer.
- Specific stimulation of simian virus 40 late transcription in vitro by a cellular factor binding the simian virus 40 21-base-pair repeat promoter element.
- Stimulation of in vitro transcription from the SV40 early promoter by the enhancer involves a specific trans-acting factor.
