Structural and Immunochemical Studies on the Phytotoxic Peptidorhamnomannan of Ceratocystis ulmi1
AUTOR(ES)
Nordin, John H.
RESUMO
A wilt-inducing peptidorhamnomannan produced by Ceratocystis ulmi, the causative agent in Dutch Elm disease, has been subjected to additional chemical and physical characterization. Gel filtration, reductive β elimination, hydrofluoric acid deglycosylation, and ultracentrifugation experiments provide evidence that the wilt-inducing polymer is polydisperse with a molecular weight range of approximately 105,000 to 120,000. The carbohydrate portion of each molecule is composed of small percentages of mannose, mannobiose, mannotriose, and a tetra- or pentasaccharide composed of mannose and rhamnose plus a major component consisting of two or three long rhamnomannan chains each with a molecular weight range of 32,000 to 34,000. All saccharide units are attached via O-glycosidic linkages to a protein with a molecular weight of approximately 35,000.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=425863Documentos Relacionados
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