Structural insights into the early steps of receptor–transducer signal transfer in archaeal phototaxis
AUTOR(ES)
Wegener, Ansgar-A.
FONTE
Oxford University Press
RESUMO
Electron paramagnetic resonance-based inter-residue distance measurements between site-directed spin-labelled sites of sensory rhodopsin II (NpSRII) and its transducer NpHtrII from Natronobacterium pharaonis revealed a 2:2 complex with 2-fold symmetry. The core of the complex is formed by the four transmembrane helices of a transducer dimer. Upon light excitation, the previously reported flap-like movement of helix F of NpSRII induces a conformational change in the transmembrane domain of the transducer. The inter-residue distance changes determined provide strong evidence for a rotary motion of the second transmembrane helix of the transducer. This helix rotation becomes uncoupled from changes in the receptor during the last step of the photocycle.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=125640Documentos Relacionados
- The genome of Nanoarchaeum equitans: Insights into early archaeal evolution and derived parasitism
- Critical steps in the early evolution of the isocortex: Insights from developmental biology
- New Insights into the Early Steps of Phosphatidylinositol Mannoside Biosynthesis in Mycobacteria: PimB′ IS AN ESSENTIAL ENZYME OF MYCOBACTERIUM SMEGMATIS*
- A receptor tyrosine kinase from choanoflagellates: Molecular insights into early animal evolution
- Early Steps of Polyomavirus Entry into Cells