Structure and expression of STK, a src-related gene in the simple metazoan Hydra attenuata.
AUTOR(ES)
Bosch, T C
RESUMO
Both cDNA clones and a genomic DNA clone encoding a 509-amino-acid protein that is 64% similar to chicken pp60c-src were isolated from the simple metazoan Hydra attenuata. We have designated this gene STK, for src-type kinase. Features of the amino acid sequence of the protein encoded by the STK gene suggest that it is likely to be myristoylated and regulated by phosphorylation in a manner similar to that found for pp60c-src. The genomic sequence encoding the protein was found to be interrupted by at least two introns, one of which was located in a position identical to that of one of the introns in the chicken src gene. The STK gene was expressed during early development of H. attenuata and at high levels in the epithelial cells of adult polyps. Probing of Hydra proteins with an antibody to phosphotyrosine indicated that the major phosphotyrosine-containing protein in H. attenuata may be the STK protein itself. H. attenuata is the simplest organism from which a protein-tyrosine kinase gene has been isolated. The presence of such a gene in the evolutionarily ancient phylum Cnidaria suggests that protein-tyrosine kinase genes arose concomitantly with or shortly after the appearance of multicellular organisms.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=362492Documentos Relacionados
- Acquisition of transforming properties by FYN, a normal SRC-related human gene.
- Maternal inheritance of transcripts from three Drosophila src-related genes.
- Isolation and sequence of a cDNA corresponding to a src-related gene expressed in murine hemopoietic cells.
- Anti-immunoglobulin stimulation of B lymphocytes activates src-related protein-tyrosine kinases.
- Polyomavirus middle-T antigen associates with the kinase domain of Src-related tyrosine kinases.