Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.

AUTOR(ES)

Gallwitz, D

RESUMO

The complete nucleotide sequence of the actin gene from Saccharomyces cerevisiae has been determined. The coding region is interrupted by a 304-base-pair intervening sequence that is located within the triplet coding for amino acid 4. DNA sequences of the intron-exon junctions are similar to those found in higher eukaryotes and can be aligned such that the intron starts with the dinucleotide 5'-G-T-3' and ends with 5'-A-G-3'. Regions fo homology within the sequences upstream from the initiation codon and those following the termination codon have been detected between the yeast iso-1-cytochrome c gene and the actin gene. As deduced from the nucleotide sequence, yeast actin has 374 amino acid residues. Its primary structure, especially the NH2-terminal third of the protein, is highly conserved during evolution.

Documentos Relacionados

• The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae.
• Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae.
• Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
• Complete nucleotide sequence of the gene encoding the regulatory subunit of 3',5'-cyclic AMP-dependent protein kinase from the yeast Saccharomyces cerevisiae.
• Nucleotide sequence of the MET8 gene of Saccharomyces cerevisiae.