Studies on Immunity in Anthrax XII. Requirement for Phosphate for Elaboration of Protective Antigen and Its Partial Replacement by Charcoal
AUTOR(ES)
Wright, George G.
RESUMO
Retention of protective antigen on columns of diethylaminoethyl cellulose was inhibited by the 0.01 m phosphate salts in the standard growth medium. Reduction in the concentration of phosphate to 0.001 m allowed satisfactory retention of antigen on diethylaminoethyl cellulose but decreased significantly the elaboration of antigen during growth of the cultures. Growth remained normal at phosphate concentrations as low as 0.0001 m. Inhibition of antigen elaboration in media containing reduced concentrations of phosphate was overcome by addition of 0.006% (w/v) charcoal. It is suggested that antigen elaboration, like encapsulation of virulent strains, is stimulated in the presence of an adsorbent. However, a nonencapsulated strain derived from an F mutant exhibited the same requirement for an adsorbent as nonencapsulated strains derived from the parent strain.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=416090Documentos Relacionados
- STUDIES ON IMMUNITY IN ANTHRAX: VII. Carbohydrate Metabolism ofBacillus anthracisin Relation to Elaboration of Protective Antigen
- STUDIES ON IMMUNITY IN ANTHRAX XI. : Control of Cellular Permeability by Bicarbonate Ion in Relation to Protective Antigen Elaboration
- STUDIES ON IMMUNITY IN ANTHRAX IX. : Effect of Variations in Cultural Conditions on Elaboration of Protective Antigen by Strains of Bacillus anthracis
- STUDIES ON IMMUNITY IN ANTHRAX IV. : Factors Influencing Elaboration of the Protective Antigen of Bacillus anthracis in Chemically Defined Media
- STUDIES ON IMMUNITY IN ANTHRAX X. : Gel-Adsorbed Protective Antigen for Immunization of Man