Studies on In Vitro DNA Synthesis Isolation of dna B Gene Product from Escherichia coli*
AUTOR(ES)
Wright, Michel
RESUMO
Formation of duplex DNA from ϕX174 single-stranded DNA by extracts of E. coli was previously shown to require the gene product of dna B. Using as an assay the stimulation of ϕX174 DNA-dependent synthesis in inactivated extracts of dna B temperature-sensitive cells, we purified the dna B gene product from wild-type E. coli as well as from a dna B temperature-sensitive mutant. The dna B temperature-sensitive gene product is more thermolabile than its wild-type counterpart.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=427183Documentos Relacionados
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