Studies on Spinach Chloroplast and Nuclear DNA Using Large-scale Tissue Preparations 1

AUTOR(ES)

Bard, Stephen A.

RESUMO

A method for the large-scale preparation of spinach chloroplasts using the Spinco Model L-4 zonal ultracentrifuge and for the extraction of DNA from the chloroplasts is described. Thirty-five percent of the chloroplast DNA (ρ = 1.706 g/cc) differs from nuclear DNA (ρ = 1.695 g/cc) in buoyant density. Tm, base composition, and renaturation properties. Sixty-five percent of the chloroplast DNA (ρ = 1.696 g/cc) has the same buoyant density and Tm as nuclear DNA, but it differs in base composition and renaturation properties.

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