Studies on the Bacteriophage 2 Receptors of Pseudomonas aeruginosa

AUTOR(ES)

Castillo, Francisco J.

RESUMO

The lysogenization of Pseudomonas aeruginosa strain BI with phage 2 resulted in the loss of the capacity to adsorb the same phage. The absence of phage 2 receptors on the surface of the lysogenized strain BI(2)8 was confirmed by the failure of purified slime polysaccharide (SPB) or lipopolysaccharide (LPS) to inactivate phage 2. SPB and LPS from a phage 2-resistant strain also failed to inactivate phage 2 in contrast to the phage inactivation exhibited by the SPB and LPS obtained from the wild-type strain BI. Chemically, quantitative differences were apparent when the SPB and LPS of strains BI(2)8 and BI/2S2 were compared with those of the wild-type strain BI. The most striking difference noted was the absence of amino sugars in the SPB of strain BI/2S2. The SPB of strain BI(2)8 also contained a lower percentage of amino sugars compared with the SPB of the wild-type strain BI.

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