STUDIES ON THE CARBOHYDRATE METABOLISM OF A GRAM-NEGATIVE ANAEROBE (BACTEROIDES SYMBIOSUS) USED IN THE CULTURE OF ENTAMOEBA HISTOLYTICA1
AUTOR(ES)
Bragg, P. D.
RESUMO
Bragg, P. D. (Louisiana State University, New Orleans) and R. E. Reeves. Studies on the carbohydrate metabolism of a gram-negative anaerobe (Bacteroides symbiosus) used in the culture of Entamoeba histolytica. J. Bacteriol. 83:76–84. 1962—Resting cells of Bacteroides symbiosus have been shown to utilize glucose and several other monosaccharides. The fermentation of the sugars is mediated by demonstrable kinases except in the case of mannitol. The main end products of metabolism of glucose are CO2, H2, ethanol, and acetic, butyric, succinic, and lactic acids. Changes in the thiol used in the growth media produce different enzyme complements in the cells. Thus, cells grown with cysteine as the thiol are unable to metabolize glucosamine, whereas those grown with thiomalate rapidly degrade the amino sugar. The results of the enzyme assay and the results from experiments with C14-labelled glucose suggest that glucose is metabolized by resting cells mainly by the Embden-Myerhof pathway.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=314791Documentos Relacionados
- Gram-Negative Bacilli of the Genus Bacteroides1
- The Gram-negative Bacteroides of the Intestine
- Acetic Acid Inhibition of Gram-negative Bacilli in Culture Media
- Concordance of endotoxemia with gram-negative bacteremia in patients with gram-negative sepsis: a meta-analysis.
- Methods for Detecting Carbohydrate Fermentation by Gram-Negative Nonsporeforming Anaerobes