STUDIES ON THE METABOLIC FUNCTION OF BRANCHED-CHAIN VOLATILE FATTY ACIDS, GROWTH FACTORS FOR RUMINOCOCCI I. : Incorporation of Isovalerate into Leucine
AUTOR(ES)
Allison, Milton J.
RESUMO
Allison, Milton J. (Dairy Cattle Research Branch, U. S. Department of Agriculture, Beltsville, Md.), M. P. Bryant, and R. N. Doetsch. Studies on the metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. I. Incorporation of isovalerate into leucine. J. Bacteriol. 83:523–532. 1962.—Ruminococcus flavefaciens strain C94, a cellulolytic rumen bacterium, requires either isobutyrate or isovalerate for growth. The organism was grown in the presence of C14-labeled isovalerate, and the metabolic fate of the labeled carbon was studied to obtain information on the functions of this growth factor. Radioactivity from isovalerate-1-C14 and isovalerate-3-C14 was found mainly in the protein and lipid fractions of the cells. The C14 in protein was all in leucine, indicating that a function of isovalerate was to serve as a carbon skeleton for leucine synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=279306Documentos Relacionados
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