Studies on the Recombination Genes of Bacteriophage T4: Suppression of uvsX and uvsY Mutations by uvsW Mutations
AUTOR(ES)
Yonesaki, Tetsuro
RESUMO
Genes uvsW, uvsX and uvsY are dispensable for T4 growth but are implicated in recombination and in the repair of damaged DNA. We found that large-plaque mutants arose efficiently from small-plaque uvsX and uvsY mutants at 42° and were pseudorevertants containing a new mutation in uvsW. Using reconstructed double mutants, we confirmed that a mutation in uvsW partially increases the burst size and UV resistance of uvsX and uvsY mutants. At 41° the uvsW mutation completely restores the arrest in DNA synthesis caused by mutations in genes uvsX, uvsY and 46, but at 30° it only partially restores DNA synthesis in a gene 46 mutant and does not restore DNA synthesis in uvsX and uvsY mutants. Restored DNA synthesis at 41° was paralleled by the overproduction of single-stranded DNA and gene 32 protein. Based on these findings, we propose that the uvsW gene regulates the production of single-stranded DNA and we discuss the phenotype of uvsW mutants and their suppression of some uvsX and uvsY phenotypes. Infection of restrictive cells with am uvsW mutants revealed a defect in the synthesis of a protein of molecular weight 53,000 daltons, suggesting that this protein is the uvsW gene product.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1203069Documentos Relacionados
- Genetic analysis of dar, uvsW, and uvsY in bacteriophage T4: dar and uvsW are alleles.
- Isolation and Characterization of Conditional Alleles of Bacteriophage T4 Genes uvsX and uvsY
- Expression of cloned bacteriophage T4 uvsW and uvsY genes in rec+ and rec- Escherichia coli.
- Confirmation of the reading frame of bacteriophage T4 uvsY gene.
- Sequence and Transcripts of the Bacteriophage T4 DNA Repair Gene uvsY