Study of microbiological and cellular tests on milk samples from buffalo females (Bubalus bubalis) in State of São Paulo / Estudo de provas microbiológicas e celulares em amostras de leite provenientes de fêmeas bubalinas (Bubalus bubalis) no Estado de São Paulo

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

The aim of the present study was the microbiological analysis of milk samples from female buffaloes, the amount evaluation of CFU/mL of the isolated microorganisms and the somatic cells counting/mL. The mammary quarters were submitted to strip cup test and CMT and 262 milk samples were colletected from buffalo cows to microbiological analysis, CFU/mL quantification and somatic cells counting. The somatic cells countings were obtained through optical microscopy reading of the milk smears, where all fields of the 1 cm2 smears area were considered. In the strip cup test, 99,6% of the samples had negative results. In the CMT, the negative results were observed in 88,2% of the samples; traces (8%), 1+ positive (1,5%), 2+ positive (1,15%) and 3+ positive (1,15%). The microbiological analysis resulted in samples with no microorganism growth (75,6%); Staphylococcus spp (11,8%); Corynebacterium spp (7,3%) and Streptococcus spp (3,1%) and associated microorganisms growth (1,15%). The total counting of somatic cells in the evaluated milk samples showed median of 2300 cells/mL; while PMN and MN counting median were 700 cells/mL and 1500 cells/mL respectivelly, with a significant statisticall difference (P<0,0001). The total somatic cells counting/mL and the amount of PMN and MN cells in the microbiological negative samples were statistically smaller than the findings in the samples that showed microorganism isolation. Concerning the amount of CFU/mL, the median obtained for samples with Corynebacterium spp was 550CFU/mL; 500 CFU/mL for Staphylococcus spp samples and 1100 CFU/mL for Streptococcus spp results, with no statiscally significant difference between them. It follows that he buffalo species has a low rate of mastitis; the number of CFU/mL is small in the microbiological positive samples. There are a number of animals that despite presenting milk microbiological exam with positive results, showed no signs of inflamatory process.

ASSUNTO(S)

células somáticas milk animal mastitis leite mastite animal buffalo búfalos somatic cells

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