Substrate Ambiguity of 3-Deoxy-d-manno-Octulosonate 8-Phosphate Synthase from Neisseria gonorrhoeae Revisited
AUTOR(ES)
Sheflyan, Galina Ya
FONTE
American Society for Microbiology
RESUMO
Homogeneous, recombinant 3-deoxy-d-manno-octulosonate 8-phosphate synthase from Neisseria gonorrhoeae is shown to catalyze the formation of 3-deoxy-d-manno-octulosonate 8-phosphate from phosphoenolpyruvate and d-arabinose 5-phosphate as determined from 1H-nuclear magnetic resonance analysis of the product. This enzyme does not catalyze the condensation of d-erythrose 4-phosphate and phosphoenolpyruvate to form 3-deoxy-d-ribo-heptulosonate 7-phosphate, as was previously reported (P. S. Subramaniam, G. Xie, T. Xia, and R. A. Jensen, J. Bacteriol. 180:119–127, 1998).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=111385Documentos Relacionados
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