Subunit structure of factor VIII antigen synthesized by cultured human endothelial cells.
AUTOR(ES)
Jaffe, E A
RESUMO
Cultured human endothelial cells were labeled with (3H)leucine, and the radioactive Factor VIII antigen present in the postculture medium was isolated by double anitbody immunoprecipitation and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after reduction with dithiothreitol. The Factor VIII antigen synthesized by cultured endothelial cells was found to contain the same single polypeptide subunit (mol wt 225,000) present in plasma Factor VIII antigen. These results suggest that in vivo, the endothelial cell is a major site of synthesis of circulating Factor VIII antigen.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=301918Documentos Relacionados
- Synthesis of Antihemophilic Factor Antigen by Cultured Human Endothelial Cells
- Downregulation of vascular endothelial growth factor receptors by tumor necrosis factor-alpha in cultured human vascular endothelial cells.
- A transferable, beta-naphthoflavone-inducible, hyperpolarizing factor is synthesized by native and cultured porcine coronary endothelial cells.
- Cyclosporine-induced synthesis of endothelin by cultured human endothelial cells.
- Synthesis of von Willebrand Factor by Cultured Human Endothelial Cells