Sulfhydryl Group Involvement in Plasmalemma Transport of HCO3− and OH− in Chara corallina1
AUTOR(ES)
Lucas, William J.
RESUMO
The effect of the sulfhydryl reagents (—SH) p-chloromercuribenzene-sulfonic acid (PCMBS), N-ethylmaleimide (NEM), and inorganic mercury on H14CO3− assimilation in Chara corallina is reported. Commercial grade PCMBS caused severe inhibition of H14CO3− assimilation. Results obtained using purified PCMBS (stock solution passed through a chelating resin) indicated that inhibition observed using unpurified PCMBS was due predominantly to the presence of inorganic mercury (as a contaminant). The inhibitory role of inorganic mercury was verified using HgCl2. This chemical caused a dramatic inhibition of H14CO3− assimilation, while it had little effect on cellular 14CO2 fixation. Reversal of the Hg2+ inhibition of H14CO3− assimilation (in presence of 1.0 millimolar dithioerythritol) was extremely slow, requiring 2 to 3 hours for the reestablishment of control rates. This slow recovery may reflect de novo synthesis of transport proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=440310Documentos Relacionados
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