Superficial and Deep Juxtaglomerular Apparatus Renin Activity of the Rat Kidney EFFECT OF SURGICAL PREPARATION AND NaCl INTAKE

AUTOR(ES)
RESUMO

The intrarenal gradient of renin activity was determined in rats by using superficial (S) and deep (D) cortical juxtaglomerular apparatuses (JGA's), identified and microdissected after silicone-rubber compound injection. Angiotensin generated from single JGA's using partially purified sheep renin substrate was quantified by rat bioassay. When, in rats on a normal NaCl diet, silicone-rubber was injected into a carotid artery, alone or with abdominal aorta catheterization, S:D renin activity ratios were 1.18±0.08 (SEM) and 1.21±0.12, respectively. The S:D renin activity ratios obtained when silicone-rubber was injected into the abdominal aorta (2.52±0.09) or a chronic carotid artery catheter (3.44±0.40) were significantly higher (P < 0.001). The lower S:D renin activity ratios after carotid artery manipulation were due to significantly higher D-JGA renin activities. This increased D-JGA renin activity and the lack of a renin gradient appear to be related to acute carotid artery manipulation.

ACESSO AO ARTIGO

Documentos Relacionados