Suppression of a Mutation in Gene 3 of Bacteriophage T7 (T7 Endonuclease I) by Mutations in Phage and Host Polynucleotide Ligase

AUTOR(ES)

Sadowski, Paul D.

RESUMO

Bacteriophage T7 bearing amber mutations in both gene 1.3 (T7 DNA ligase) and gene 3 (T7 endonuclease I) are viable when grown in suppressor-negative, ligase-negative hosts. This is evidenced by a high plating efficiency and a large burst size compared to the single mutants. These findings may be explained by a limited destruction of cellular DNA by the double mutant.

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