Surface properties of Chlamydia psittaci.

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RESUMO

The surface properties of elementary bodies of Chlamydia psittaci were investigated by diethylaminoethyl-Sephadex chromatography, cytophoresis, partitioning in an aqueous polymer two-phase system, and hydrophobic interaction chromatography of the organism. The surface of C. psittaci was found to be hydrophobic and negatively charged at pH 7 and to have an isoelectric point of about pH 5. Reagents which block free carboxyl groups altered the surface charge of C. psittaci and caused the organism to agglomerate. The possible significance of hydrophobicity and surface charge on the ingestion of C. psittaci by host cells is discussed.

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