Survival of tubercle bacilli in heat-fixed and stained sputum smears
AUTOR(ES)
Cardoso, Celso Luíz, Giacomelli, Luiz Roberto Bigão, Helbel, César, Sant'Ana, Janete Jordão, Martins, Fátima Moreira, Barreto, Angela Maria Werneck
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2001-02
RESUMO
We used a slide culture technique to detect tubercle bacilli surviving in sputum smears (n=46) after conventional heat fixation and Ziehl-Neelsen staining. In all heat-fixed sputum smears, tubercle bacilli survived after time 0 (n=22), 24 h (n=7), 48 h (n=7), 72 h (n=4), and seven days (n=6). None of the stained sputum smears showed growth on slide cultures. Viable tubercle bacilli remaining in heat-fixed sputum smears for at least seven days may present an infection risk to laboratory staff. Thus, sputum smears should be stained immediately by the Ziehl-Neelsen method or stored in a safe container to avoid transmission of tuberculosis.
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