SwaI, a unique restriction endonuclease from Staphylococcus warneri, which recognizes 5'-ATTTAAAT-3'.
AUTOR(ES)
Lechner, M
RESUMO
A novel class-II restriction endonuclease designated SwaI was purified from Staphylococcus warneri. This enzyme cleaves adenovirus 2 DNA, SV40 DNA and M13mp7 at one site each, but does not cleave lambda, PhiX174, pBR322 or pBR328 DNA. SwaI recognizes the octanucleotide sequence 5'-ATTTAAAT-3', cleaving in the center of the recognition sequence creating blunt ended DNA fragments. SwaI was used to digest chromosomal DNA from various microorganisms and human cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=312344Documentos Relacionados
- AciI, a unique restriction endonuclease from Arthrobacter citreus which recognizes 5' CCGC 3'.
- BsrI, a unique restriction endonuclease from Bacillus stearothermophilus which recognizes 5' ACTGG3'.
- ApoI, a unique restriction endonuclease from Arthrobacter protophormiae which recognizes 5' RAATTY 3'.
- DrdI, a unique restriction endonuclease from Deinococcus radiodurans which recognizes 5'GACN6GTC3'.
- Mse I, a unique restriction endonuclease from Micrococcus species which recognizes 5' T decreases TAA 3'.
