SYNTHESIS AND INDUCTION OF TYROSINE AMINOTRANSFERASE IN SYNCHRONIZED HEPATOMA CELLS IN CULTURE*

AUTOR(ES)

Martin, David

RESUMO

The generation cycle of an established line of rat hepatoma cells (HTC cells) was studied using synchronized cell techniques. Dexamethasone phosphate (Dex), a synthetic adrenal corticosteriod which induces tyrosine aminotransferase (TAT) in HTC cells randomly distributed in the cell generation cycle, did not affect the durations of the various phases of the cycle. The activities of TAT and several dehydrogenases, and the rates of general protein and RNA synthesis, were studied throughout the cycle.

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