Synthesis and Secretion of Hypoxyproline-containing Macromolecules in Carrots: III. Metabolic Requirements for Secretion 1

AUTOR(ES)

Doerschug, Marcia R.

RESUMO

Using pulse-chase experiments with radioactive proline, it is possible to study the rapid transfer from the cytoplasm to the cell wall of the hydroxyproline-rich protein found in the cell walls of higher plants. The secretion of this protein is not obligatorily coupled to protein synthesis. Secretion is completely inhibited by uncouplers of oxidative phosphorylation and strongly inhibited by the inhibitors of electron transport, cyanide and azide. It is concluded that the transfer of proteins from the cytoplasm to the cell wall is an energy-requiring step.

Documentos Relacionados

• Synthesis and Secretion of Hydroxyproline-containing Macromolecules in Carrots: II. In vivo Conversion of Peptidyl Proline to Peptidyl Hydroxyproline
• Synthesis and Secretion of Hydroxyproline Containing Macromolecules in Carrots. I. Kinetic Analysis
• TRYPTOPHANASE-TRYPTOPHAN SYNTHETASE SYSTEMS IN ESCHERICHIA COLI III. : Requirements for Enzyme Synthesis
• IMMUNOGLOBULIN SYNTHESIS AND SECRETION, III. INCORPORATION OF GLUCOSAMINE INTO IMMUNOGLOBULIN ON POLYRIBOSOMES*
• Growth Factors For Bacteria: III. Some Nutritive Requirements of Lactobacillus delbrucki1