Synthesis of two collagen types by embryonic chick corneal epithelium in vitro.
AUTOR(ES)
Linsenmayer, T F
RESUMO
To better understand the mechanisms involved in matrix development, we have analyzed the collagen synthesized by embryonic corneal epithelium, the tissue known to produce the collagenous component of the primary corneal stroma. Isolated epithelia were cultured in vitro in medium containing [oH]proline and the newly synthesized, labeled collagen was extracted, fractionally salt precipitated, and analyzed by carboxymethyl-cellulose chromatography and peptide mapping after cleavage with cyanogen bromide. The data show that the cornela epithelium produces at least two different types of collagen, one similar, if not identical, to the type I molecule of skin, and a second similar, if not identical, to the type II molecule of cartilage. Type II, heretofore, had been thought to be characteristic of cartilage extracellular matrix.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393192Documentos Relacionados
- Synthesis of type II collagen in vitro by embryonic chick neural retina tissue.
- Effects of temperature, amebic strain, and carbohydrates on Acanthamoeba adherence to corneal epithelium in vitro.
- In vitro synthesis of native myosin, actin, and tropomyosin from embryonic chick polyribosomes.
- Functional expression of A-currents in embryonic chick sympathetic neurones during development in situ and in vitro.
- Collagen Synthesis: A Disulfide-Linked Collagen Precursor in Chick Bone*