Synthesis of U1 RNA in isolated nuclei from sea urchin embryos: U1 RNA is initiated at the first nucleotide of the RNA.
AUTOR(ES)
Morris, G F
RESUMO
Nuclei from sea urchin blastula embryos synthesize a variety of small RNAs, one of which has identical mobility with sea urchin U1 RNA. This RNA is synthesized by RNA polymerase II and, in a hybridization-selection experiment, was selected by the cloned sea urchin U1 gene. The U1 RNA was initiated with ATP, but not GTP, in isolated nuclei with beta-S- and gamma-S-ribonucleotide triphosphates as substrates. The U1 RNA containing thiophosphate at the 5' end was not capped but accumulated as an uncapped transcript from which the thiophosphate could be removed with calf intestinal phosphatase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=366833Documentos Relacionados
- Synthesis of U1 RNA in a DNA-dependent system from sea urchin embryos.
- Structure of the sea urchin U1 RNA repeat.
- ENZYMES OF DNA SYNTHESIS IN NUCLEI OF SEA URCHIN EMBRYOS*
- Synthesis of U1 RNA in isolated mouse cell nuclei: initiation and 3'-end formation.
- Expression of the U1 RNA gene repeat during early sea urchin development: evidence for a switch in U1 RNA genes during development.