Synthesis, secretion and processing of alpha-factor-interferon fusion proteins in yeast.

AUTOR(ES)

Singh, A

RESUMO

A gene fusion consisting of 960 base pairs of 5'-flanking region of the yeast MF alpha 1 gene, 257 base pairs coding for alpha-factor prepro sequence, and a modified human IFN-alpha 1 gene was constructed. MAT alpha cells containing the chimeric gene synthesized and secreted active IFN-alpha 1 into the growth medium. The secreted interferon molecules contained the last 4 amino acids of alpha-factor prepro sequence and the amino acids encoded by the DNA modifications introduced at the beginning of IFN-alpha 1 gene. DNA sequences coding for these amino acids were removed by oligonucleotide-directed in vitro mutagenesis. Yeast cells transformed with expression plasmids containing the altered junction synthesized and secreted human IFN-alpha 1 with the natural NH2-terminus.

Documentos Relacionados

• Secretion and processing of insulin precursors in yeast.
• An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast.
• Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.
• Synthesis, processing, and secretion of the Marek's disease herpesvirus A antigen glycoprotein.
• Submitochondrial localization, cell-free synthesis, and mitochondrial import of 2-isopropylmalate synthase of yeast.