T cell ignorance in mice to Borna disease virus can be overcome by peripheral expression of the viral nucleoprotein
AUTOR(ES)
Hausmann, Jürgen
FONTE
The National Academy of Sciences
RESUMO
Infection of neonates with Borna disease virus (BDV) induces severe meningoencephalitis and neurological disorder in wild-type but not in β2-microglobulin-deficient mice of strain MRL (H-2k). Temporary in vivo depletion of CD8+ T cells delayed BDV-induced disease for several weeks. Depletion of CD4+ T cells had a similar beneficial effect, indicating that the BDV-induced neurological disorder in mice is a CD4+ T cell-dependent immunopathological process that is mediated by CD8+ T cells. Lymphocytes prepared from brains of diseased mice were mainly from the CD8+ T cell subset. They showed up-regulation of activation markers and exerted strong MHC I-restricted cytotoxic activity against target cells expressing the BDV nucleoprotein p40. Infection of B10.BR (H-2k) or congenic C57BL/10 (H-2b) mice resulted in symptomless, lifelong persistence of BDV in the brain. Superinfection with a recombinant vaccinia virus expressing BDV p40 but not with other vaccinia viruses induced severe neurological disease and encephalitis in persistently infected B10.BR mice but not in persistently infected C57BL/10 mice, indicating that the disease-inducing T cell response is restricted to the nucleoprotein of BDV in H-2k mice. Our results demonstrate that the cellular arm of the immune system may ignore the presence of a replicating virus in the central nervous system until proper antigenic stimulation at a peripheral site triggers the antiviral response.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=22285Documentos Relacionados
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