T-cell modulation of the murine antibody response to Neisseria meningitidis group A capsular polysaccharide.
AUTOR(ES)
Muller, E
RESUMO
T-cell modulation of the antibody response of BALB/c mice to group A meningococcal capsular polysaccharide (PS) was examined by using an enzyme-linked immunosorbent assay. An optimal dose (5 micrograms) of antigen induced an immunoglobulin M (IgM) response of short duration; no IgG or IgA antibody could be detected. The capacity to produce serum antibody begins at about 3 weeks of age. Concanavalin A (ConA) inhibited the magnitude of the response by 40 to 60% when given at the time of immunization; it enhanced the response two- to eightfold when given 2 days after PS. T-cell-mediated suppression could be transferred to naive mice by injection of spleen cells from low-dose-primed mice. A secondary antibody response could be induced by immunization with live meningococci. Here, the IgM response was 8- to 10-fold greater than that of mice given an optimal dose of PS; IgG antibody against group A PS increased 1 week after immunization to levels that were 100- to 1,000-fold greater than those of mice immunized with PS. The antibody response could not be augmented by multiple injections of PS; suppression occurred after low-dose priming or hyperimmunization with PS. These studies indicate that the antibody response to PS is not completely T-cell independent; rather, it is inhibited and amplified by T cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=259266Documentos Relacionados
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