Tandem MS analysis of brain clathrin-coated vesicles reveals their critical involvement in synaptic vesicle recycling
AUTOR(ES)
Blondeau, Francois
FONTE
National Academy of Sciences
RESUMO
Tandem MS has identified 209 proteins of clathrin-coated vesicles (CCVs) isolated from rat brain. An overwhelming abundance of peptides were assigned to the clathrin coat with a 1:1 stoichiometry observed for clathrin heavy and light chains and a 2:1 stoichiometry of clathrin heavy chain with clathrin adaptor protein heterotetramers. Thirty-two proteins representing many of the known components of synaptic vesicles (SVs) were identified, supporting that a main function for brain CCVs is to recapture SVs after exocytosis. A ratio of vesicle-N-ethylmaleimide-sensitive factor attachment protein receptors to target-N-ethylmaleimide-sensitive factor attachment protein receptors, similar to that previously detected on SVs, supports a single-step model for SV sorting during CCV-mediated recycling of SVs. The uncovering of eight previously undescribed proteins, four of which have to date been linked to clathrin-mediated trafficking, further attests to the value of the current organelle-based proteomics strategy.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=374330Documentos Relacionados
- Dynamin-dependent Transferrin Receptor Recycling by Endosome-derived Clathrin-coated Vesicles
- Topological mechanisms involved in the formation of clathrin-coated vesicles.
- Clathrin-coated vesicles contain an ATP-dependent proton pump.
- Association of the fusion protein NSF with clathrin-coated vesicle membranes.
- Uncoating of clathrin-coated vesicles by uncoating ATPase from developing peas.