Targeting deletion (homoeologous chromosome pairing locus) or addition line single copy sequences from cereal genomes.

AUTOR(ES)

Clarke, B

RESUMO

We describe here a protocol for obtaining clones containing sequences present in low copy-number from genomic DNA where moderately and highly repeated sequences predominate. Specific chromosomal regions can be targeted by using deletion or addition line material. We have used this protocol to identify a sequence which has been deleted in both the tetraploid and hexaploid wheat mutants for the homoeologous chromosome pairing locus.

Documentos Relacionados

• Insights into cereal genomes from two draft genome sequences of rice
• Highly repetitive DNA sequences in cyanobacterial genomes.
• Discrete-length repeated sequences in eukaryotic genomes.
• Ubiquitous, interspersed repeated sequences in mammalian genomes.
• Short, interspersed repetitive DNA sequences in prokaryotic genomes.