Telomeric D-loops Containing 8-Oxo-2′-deoxyguanosine Are Preferred Substrates for Werner and Bloom Syndrome Helicases and Are Bound by POT1*
AUTOR(ES)
Ghosh, Avik
FONTE
American Society for Biochemistry and Molecular Biology
RESUMO
8-Oxo-2′-deoxyguanosine (8-oxodG) is one of the most important oxidative DNA lesions, and G-rich telomeric DNA is especially susceptible to oxidative DNA damage. RecQ helicases WRN and BLM and telomere-binding protein POT1 are thought to play roles in telomere maintenance. This study examines the ability of WRN, BLM, and RecQ5 to unwind and POT1 to bind telomeric D-loops containing 8-oxodG. The results demonstrate that WRN and BLM preferentially unwind telomeric D-loops containing 8-oxodG and that POT1 binds with higher affinity to telomeric D-loops with 8-oxodG but shows no preference for telomeric single-stranded DNA with 8-oxodG. We speculate that telomeric D-loops with 8-oxodG may have a greater tendency to form G-quadruplex DNA structures than telomeric DNA lacking 8-oxodG.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2781507Documentos Relacionados
- Preferential cleavage of plasmid-based R-loops and D-loops by Drosophila topoisomerase IIIβ
- The Bloom’s and Werner’s syndrome proteins are DNA structure-specific helicases
- D-loops and R-loops: alternative mechanisms for the initiation of chromosome replication in Escherichia coli.
- Regulation of mitochondrial D-loops by transcription factor A and single-stranded DNA-binding protein
- A novel assay of 8-oxo-2'-deoxyguanosine 5'-triphosphate pyrophosphohydrolase (8-oxo-dGTPase) activity in cultured cells and its use for evaluation of cadmium(II) inhibition of this activity.
