Temperature-Sensitive Modification and Restriction Phenotypes of an Escherichia coli dnaD Mutant
AUTOR(ES)
Paul, Aniko V.
RESUMO
A mutant of Escherichia coli temperature-sensitive for deoxyribonucleic acid synthesis, dnaD, was found to have temperature-sensitive modification and restriction phenotypes. In contrast to the original observation by Carl (1970), the mutant could support the growth of λ phage at 41 C. However, the λ phages thus produced were able to form plaques with normal plating efficiency only on E. coli C, a restriction-less strain, but not on E. coli K. Since the λ phages produced in the mutant at 30 C could form plaques equally well on both E. coli strains, it was concluded that the dnaD mutant has a temperature-sensitive modification phenotype. Furthermore, since the dnaD mutant allowed some growth of unmodified λ·C phages at 41 C but less at 30 C, the mutant is also temperature sensitive in restriction. The relationship, if any, between temperature-sensitive deoxyribonucleic acid synthesis and temperature-sensitive modification-restriction in the dnaD mutant is not known. Similar experiments were done with three dnaC mutants and one dnaA mutant. Two dnaC mutants were found to have altered restriction phenotypes at 41 C, but none of the mutants were defective in modification.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=245698Documentos Relacionados
- Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase.
- Genetic Characterization of the Temperature-Sensitive and Suppression Phenotypes of Escherichia coli Mutant N4316
- Genetic Characterization of the Temperature-Sensitive and Suppression Phenotypes of Escherichia coli Mutant N4316
- A TEMPERATURE-SENSITIVE STREPTOMYCIN REQUIREMENT IN AN ESCHERICHIA COLI MUTANT
- Temperature-Sensitive Mutant of Escherichia coli K-12 with an Impaired d-Alanine: d-Alanine Ligase
