Temperature sensitive mutants of Escherichia coli for tRNA synthesis
AUTOR(ES)
Sakano, Hitoshi
RESUMO
An efficient method was devised to isolate temperature sensitive mutants of E. coli defective in tRNA biosynthesis. Mutants were selected for their inability to express suppressor activity after su3+-transducing phage infection. In virtually all the mutants tested, temperature sensitive synthesis of tRNATyr was demonstrated. Electrophoretic fractionation of 32P labeled RNA synthesized at high temperature showed in some mutants changes in mobility of the main tRNA band and the appearance of slow migrating new species of RNA. Temperature sensitive function of mutant cells was also evident in tRNA synthes: directed by virulent phage T4 and BF23. We conclude that although the mutants show individual differences, many are temperature sensitive in tRNA maturation functions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=344021Documentos Relacionados
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