Temperature-Sensitive Nonsense Mutations in Essential Genes of Escherichia coli
AUTOR(ES)
Beckman, David
RESUMO
Cells containing nonsense mutations in essential genes have been isolated in a strain of Escherichia coli that carried the su4ts gene which specifies a temperature-sensitive tyrosine transfer ribonucleic acid. Such cells are unable to form colonies at temperatures which inactivate this suppressor transfer ribonucleic acid. A screening procedure for the identification of mutants that carry temperature-sensitive nonsense mutations in essential genes is described, and certain properties of two such mutants are reported.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246492Documentos Relacionados
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