Temperature-Sensitive Variants of NICOTIANA TABACUM Isolated from Somatic Cell Culture
AUTOR(ES)
Malmberg, Russell L.
RESUMO
Temperature-sensitive variants of Nicotiana tabacum were isolated from a liquid suspension culture of somatic cells by a negative selection procedure, using bromodeoxyuridine and light. A total of nine such variants have been recovered, with an estimated rate of 2 x 10-7 per cell division. The appearance of the variants at the permissive temperature varied from nearly wild type, white and friable, to brown, compact and slow growing. Two of the variants adapted from growth on solid medium to growth in a liquid suspension culture; these were further characterized for chromosome number, growth rate, cell death rate at the restrictive temperature, growth on nutritionally modified media, and RNA and protein synthesis. The variants have been placed on regeneration media, and one of them has produced plantlets. Leaves from a plantlet have been placed on callus-inducing media, and the resulting callus displayed the temperature-sensitive phenotype.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1213943Documentos Relacionados
- Extranuclear Temperature-Sensitive Lethality in Nicotiana tabacum L
- Temperature-Sensitive Variants of an Established Myoblast Line
- Temperature-sensitive mutants isolated from hamster and canine cell lines persistently infected with Newcastle disease virus.
- Two independent mutations are required for temperature-sensitive cell transformation by a Rous sarcoma virus temperature-sensitive mutant.
- Temperature-sensitive transformation by Rous sarcoma virus and temperature-sensitive protein kinase activity.