Terpene Biosynthesis in Cell-free Extracts and Excised Shoots from Wedgwood Iris 1
AUTOR(ES)
Staby, George L.
RESUMO
Excised shoots and cell-free extracts prepared from Wedgwood iris (Iris hollandica Hoog. “Wedgwood”) shoots metabolized 14C-labeled mevalonic acid (MVA). By using cell-free extracts, the 14C from MVA-1-14C was recovered as 14CO2, while that from MVA-2-14C was recovered as neutral terpenes, acid-hydrolyzable terpenes, or 14CO2. Also, under optimal incubation conditions, 12.8 nanomoles R-MVA-2-14C was incorporated into neutral terpenes per milligram fresh weight per hour. In contrast, excised shoots incorporated only 0.58 nanomoles R-MVA-2-14C per mg fresh weight per hour. Labeled products identified from the cell-free system were squalene, farnesol, geranylgeraniol, and compounds that are converted to farnesol and geranylgeraniol after alkaline hydrolysis. Squalene and a 4,4-dimethylsterol were identified as products from excised shoots but not the terpene alcohols or the alkaline-hydrolyzable compounds.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=366514Documentos Relacionados
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