The 245 base-pair oriC sequence of the E. coli chromosome directs bidirectional replication at an adjacent region.
AUTOR(ES)
Tabata, S
RESUMO
The replication origin of the E. coli K-12 chromosome has been isolated as autonomously replicating molecules(oriC plasmid), and the DNA region essential for replicating function(oriC) has been localized to a sequence of 232-245 base-pairs(bp) by deletion analysis. In this report, the functional role of oriC was analysed by using an in vitro replication system and various OriC+ and OriC- plasmids previously constructed. The results obtained were summarized as follows: (1) The oriC sequence contained information enough to direct bidirectional replication. (2) The actual DNA replication began at a region near, but outside, oriC and progressed bidirectionally. (3) Initiation of DNA synthesis at the specific region required the dnaA-complementing fraction from cells harboring a dnaA-carrying plasmid.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=325912Documentos Relacionados
- Functional analysis of minichromosome replication: bidirectional and unidirectional replication from the Escherichia coli replication origin, oriC.
- Effect of dam methylation on the activity of the E. coli replication origin, oriC.
- oriC Region and Replication Termination Site, dif, of the Xanthomonas campestris pv. campestris 17 Chromosome
- Start sites for bidirectional in vitro DNA replication inside the replication origin, oriC, of Escherichia coli.
- Repair of defined single base-pair mismatches in Escherichia coli
