The Attachment Site of Streptomycin to the 30S Ribosomal Subunit

AUTOR(ES)

Biswas, Debajit K.

RESUMO

The 16S RNA dissociated from 30S ribosomal subunits of Escherichia coli strains either sensitive or resistant to streptomycin contains the attachment sites for two streptomycin molecules, as does the undissociated particle from a streptomycin-sensitive strain. Since no streptomycin binds to undissociated 30S subunits from a streptomycin-resistant strain, it is suggested that protein P10, specified by the strA locus—known to be responsible for drug sensitivity—controls the availability to streptomycin of the attachment sites. These sites remain exposed in the strA+ wild-type, and become masked in strA streptomycin-resistant mutants.

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