The Bacillus subtilis spoIIG operon encodes both sigma E and a gene necessary for sigma E activation.

AUTOR(ES)

Jonas, R M

RESUMO

A sporulation-specific sigma factor of Bacillus subtilis (sigma E) is formed by a proteolytic activation of a precursor protein (P31). Synthesis of the precursor protein is shown to be abolished in B. subtilis mutants with plasmid insertions as far as 940 base pairs upstream of the P31 structural gene (sigE), and processing of P31 to sigma E is blocked by a deletion in this upstream region. These results substantiate the view that sigE is the distal member of a 2-gene operon and demonstrate that the upstream gene (spoIIGA) is necessary for sigma E formation.

Documentos Relacionados

• Activation of the Bacillus subtilis spoIIG promoter requires interaction of Spo0A and the sigma subunit of RNA polymerase.
• Cloning of sporulation gene spoIIG in Bacillus subtilis.
• Bacillus subtilis sigma factor sigma 29 is the product of the sporulation-essential gene spoIIG.
• A Region in the Bacillus subtilis Transcription Factor Spo0A That Is Important for spoIIG Promoter Activation
• Surfaces of Spo0A and RNA Polymerase Sigma Factor A That Interact at the spoIIG Promoter in Bacillus subtilis