The basic helix–loop–helix–PAS protein ARNT functions as a potent coactivator of estrogen receptor-dependent transcription
AUTOR(ES)
Brunnberg, Sara
FONTE
National Academy of Sciences
RESUMO
The biological effects of estrogens are mediated by the estrogen receptors ERα and ERβ. These receptors regulate gene expression through binding to DNA enhancer elements and subsequently recruiting factors such as coactivators that modulate their transcriptional activity. Here we show that ARNT (aryl hydrocarbon receptor nuclear translocator), the obligatory heterodimerization partner for the aryl hydrocarbon receptor and hypoxia inducible factor 1α, functions as a potent coactivator of ERα- and ERβ- dependent transcription. The coactivating effect of ARNT depends on physical interaction with the ERs and involves the C-terminal domain of ARNT and not the structurally conserved basic helix–loop–helix and PAS (Per-ARNT-Sim) motifs. Moreover, we show that ARNT/ER interaction requires the E2-activated ligand binding domain of ERα or ERβ. These observations, together with the previous role of ARNT as an obligatory partner protein for conditionally regulated basic helix–loop–helix–PAS proteins like the aryl hydrocarbon receptor or hypoxia inducible factor 1α, expand the cellular functions of ARNT to include regulation of ERα and ERβ transcriptional activity. ARNT was furthermore recruited to a natural ER target gene promoter in a estrogen-dependent manner, supporting a physiological role for ARNT as an ER coactivator.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=164478Documentos Relacionados
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