The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.
AUTOR(ES)
Rosa, F
RESUMO
The human Burkitt lymphoma cell line Daudi does not synthesize beta2-microglobulin (beta2m) and lacks the cell surface histocompatibility antigens. The cells, however, contain RNA hybridizing to a cloned human beta2m cDNA probe. cDNA from this Daudi beta2m RNA, was cloned and sequenced. By comparison with cDNA prepared from Ramos cells, which synthesized microglobulin, we determined the sequence of the 20 amino acid long leader peptide of pre-beta2m and show that in Daudi cells the initiator ATG has been mutated to ATC. Although Daudi beta2m RNA cannot be translated, interferon induces the beta2m RNA in Daudi cells as well as in normal human cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=555120Documentos Relacionados
- Selective reduction of c-myc mRNA in Daudi cells by human beta interferon.
- Analysis of interferon mRNA in human fibroblast cells induced to produce interferon.
- Increased rate of degradation of c-myc mRNA in interferon-treated Daudi cells.
- The 3' untranslated region of the human interferon-beta mRNA has an inhibitory effect on translation.
- Reduced beta 2-microglobulin mRNA levels in transgenic mice expressing a designed hammerhead ribozyme.